不同内吞方式中的关键因子抗体对树突状细胞捕获猪细小病毒样颗粒的影响

doi:10.11843/j.issn.0366-6964.2016.09.022

Abstract

Abstract:

Recombinant parvovirus-like particles (PPV-VLPs) are particulate exogenous antigens that induce strong CTL response.In order to decipher the mechanisms responsible for CTL activation by such exogenous antigen and the probable endocytic pathways in PPV-VLP capture，we analyzed the influence of different antibodies on dendritic cells (DCs) endocytic uptaking recombinant parvovirus-like particles.The results analyzed by FACStar cytometer and confocal microscopy showed that the PPV-VLPs capture efficiency of spleen DCs pretreated with anti-CD64 and anti-DEC205 antibodies was not affected.However，anti-caveolin-1 antibodies treated DCs was severely reduced.DCs pretreated with anti-actin antibodies were barely to uptake PPV-VLPs.These results showed that the uptake of PPV-VLPs seems to be related to macropinocytosis and caveolae-mediated endocytosis in an actin-dependent pathway，not clathrin-coated pits or phagocytosis.

CLC Number:

S852.4

SHEN Xiao-qiang，ZUO Yu-zhu，DI Jing-mei，GU Wen-yuan，FAN Jing-hui. The Influence of Antibodies against Key Factors of Different Endocytic Pathways on Dendritic Cells Uptake Recombinant Parvovirus-like Particles[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1924-1930.

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The Influence of Antibodies against Key Factors of Different Endocytic Pathways on Dendritic Cells Uptake Recombinant Parvovirus-like Particles

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